FastQC Summary

Read Totals

Library Sizes ranged between 45,380,672 and 105,067,734 reads.

Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.

FastQC Summary

Summary of FastQC flags for each parameter

Per Base Sequence Quality

Heatmap showing mean base qualities for each library

Per Sequence Quality Scores

Heatmap showing mean sequence qualities for each library

Per Base Sequence Content

Heatmap of summed base distributions along each read

Per Sequence GC Content

GC Content Heatmap normalised to theoretical GC content in the Hsapiens Transcriptome

GC Content Distributions for all reads showing theoretical GC content from the Hsapiens Transcriptome

Sequence Length Distribution

Sequence Duplication Levels

Adapter Content

Universal Adapter Content

Overrepresented Summary

Total overrepresented sequences for each library

Total overrepresented sequences for each library

Overrepresented Sequences

## Overrepresented_sequences missing from GLL50_D_S13Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL50_V_S38Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL53_V_S52Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL56_D_S25Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL56_V_S7Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL59_D_S19Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL59_V_S72Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL63_D_S15Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL63_V_S8Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL65_D_S48Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL65_V_S69Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL67_D_S22Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL67_V_S74Aligned.sortedByCoord.out.bam
## Overrepresented_sequences missing from GLL69_V_S39Aligned.sortedByCoord.out.bam

Session Information

R version 4.0.0 (2020-04-24)

Platform: x86_64-apple-darwin17.0 (64-bit)

locale: en_AU.UTF-8||en_AU.UTF-8||en_AU.UTF-8||C||en_AU.UTF-8||en_AU.UTF-8

attached base packages: parallel, stats, graphics, grDevices, utils, datasets, methods and base

other attached packages: pander(v.0.6.3), DT(v.0.13), scales(v.1.1.0), readr(v.1.3.1), dplyr(v.0.8.5), stringr(v.1.4.0), magrittr(v.1.5), ngsReports(v.1.4.0), tibble(v.3.0.1), ggplot2(v.3.3.0) and BiocGenerics(v.0.34.0)

loaded via a namespace (and not attached): Biobase(v.2.48.0), ggdendro(v.0.1-20), httr(v.1.4.1), tidyr(v.1.0.3), jsonlite(v.1.6.1), viridisLite(v.0.3.0), assertthat(v.0.2.1), highr(v.0.8), stats4(v.4.0.0), latticeExtra(v.0.6-29), GenomeInfoDbData(v.1.2.3), Rsamtools(v.2.4.0), yaml(v.2.2.1), ggrepel(v.0.8.2), pillar(v.1.4.4), lattice(v.0.20-41), glue(v.1.4.0), digest(v.0.6.25), RColorBrewer(v.1.1-2), GenomicRanges(v.1.40.0), XVector(v.0.28.0), colorspace(v.1.4-1), htmltools(v.0.4.0), Matrix(v.1.2-18), plyr(v.1.8.6), FactoMineR(v.2.3), pkgconfig(v.2.0.3), ShortRead(v.1.46.0), zlibbioc(v.1.34.0), purrr(v.0.3.4), jpeg(v.0.1-8.1), BiocParallel(v.1.22.0), farver(v.2.0.3), generics(v.0.0.2), IRanges(v.2.22.1), ellipsis(v.0.3.0), withr(v.2.2.0), SummarizedExperiment(v.1.18.1), lazyeval(v.0.2.2), crayon(v.1.3.4), evaluate(v.0.14), MASS(v.7.3-51.6), hwriter(v.1.3.2), forcats(v.0.5.0), truncnorm(v.1.0-8), Cairo(v.1.5-12), tools(v.4.0.0), data.table(v.1.12.8), hms(v.0.5.3), lifecycle(v.0.2.0), matrixStats(v.0.56.0), plotly(v.4.9.2.1), S4Vectors(v.0.26.0), munsell(v.0.5.0), cluster(v.2.1.0), DelayedArray(v.0.14.0), Biostrings(v.2.56.0), flashClust(v.1.01-2), compiler(v.4.0.0), GenomeInfoDb(v.1.24.0), rlang(v.0.4.6), grid(v.4.0.0), RCurl(v.1.98-1.2), htmlwidgets(v.1.5.1), crosstalk(v.1.1.0.1), leaps(v.3.1), labeling(v.0.3), bitops(v.1.0-6), rmarkdown(v.2.1), gtable(v.0.3.0), reshape2(v.1.4.4), R6(v.2.4.1), GenomicAlignments(v.1.24.0), zoo(v.1.8-8), lubridate(v.1.7.8), knitr(v.1.28), stringi(v.1.4.6), Rcpp(v.1.0.4.6), png(v.0.1-7), vctrs(v.0.2.4), scatterplot3d(v.0.3-41), tidyselect(v.1.0.0) and xfun(v.0.13)